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 Molecular Oncology Laboratory Protocols

     MOLab Biosafety Manual   UC Safety   E & HS  Safety Talks
     MOLab Survival Manual    Safety Online Training Modules
      MOLab "Law and Order"
     MOLab Unconventional Cloning Tips
A.  Bacterial, DNA & Cloning Techniques
B.  PCR Techniques          
C.  RNA Techniques
D.  Protein Techniques

E.  Tissue & Cell Culture Techniques  Hood Sign-up

       Cell Culture Basics Videos  V-1  V-2  V-3  V-4  V-5 

F.  Skeletal & Stem Cell Biology & Histology Techniques 
G.  Instrument Use & Procedures 
H.  Solutions, Buffers & Miscellaneous
Animal Bookkeeping:  Tumors     Bone/Stem Cells     Rats
     Molecular Oncology Lab Graduation Checklist

 MOLab Cloning and Expression Vectors

UCMOLab Vectors     MOLab Vector Template      MOLab Construct Submission Form

Adenoviral Vectors (Classical)
pAdEasyVectors pAdTrack-TOX (eGFP) pAdTrace-TOX (mRFP) pSES1 (AdV siRNA, SfiI)
pASG (AdV shuttle w/ copGFP) pASG2 (AdV w/ 2x CMV-PA) pAdTrace-TO4  (mRFP) pSES (AdV siRNA, pSES-HUS)
pShuttle-LG (LoxP-GFP) pShuttle-RFP (mRFP) pAdTrack-TO4  (eGFP)  
pAIR (Adv RheoSwitch inducible) pAdHD-Linker(helper-dependent) pAdHD-Linker2(helper-dependent)  
pAdEasy3      

MOLab One-Step (MOS), miRs, ncRNA reporter, and CRISPR Vectors

pAdGOS   pAdGOSd pMPB-OMIR pBOK (piggyBac)  pBOKd(GA- siRNAs) pSOK  pSOKd (GA-siRNAs)
pAdROS   pAdROSd pAdTrack-OMIR pAdTrace-OK pAdTrace-OKd(siRNAs) pSOK1 (w GFP) pSOK2 (w GFP)
pU2MIR (U6 template) pAdTrace-OMIR pAdTrack-OK pAdTrack-OKd(siRNAs) pB2B (template for GA-siRNAs)
pNRGLuc (ncRNA reporter) pSEB-OMIR pSOHU (retroviral, HindIII/SalI) pMPB-SOS (pMPB-SOSd, H3/RI)
pAdTrace-AMO pSEB-AMO   pSEB-SOSd (HindIII/EcoRI)
pAdTrace-miR pSEB-miR   pAdTrace-SOSd  (HindIII/EcoRI)
pSEB-CIR pSEBR-CIR    
pUCGR pU6CGR pUCC4  
MOLab Modified Lentiviral Vectors (MLVs)(3rd Generation)
pMLV-Linker      
       
       
       
       
pCDF1-MCS1-EF1-copGFP pCDF1-MCS2-EF1-copGFP pCDH-CMV-MCS-EF1-Puro  
Retroviral Vectors
pSEB-C3F (C-terminal 3x Flag) pSEB-N3F (N-terminal 3x Flag) pSEB-3H (3x 6His) pSEB-3M (3x Myc)
pSEB-HUS (Sfi I) (RV siRNA = pSOS) pBGLuc (GLuc reporter) pSEB-Linker pSEB-Flag
pSEN-Flag pSEH-Flag pSEZ-Flag pSBC-3F (CMV)
pSEH-HUS (Sfi I) (RV siRNA) pSEN-HUS (Sfi I) (RV siRNA) pSEB-HUD (Xcm I) (RV siRNA) pSEB-CG (C-terminal copGFP)
pSEB-NG (N-terminal copGFP) pSEB-CBT (C-terminal biotin tag) pSEB-NBT (N-terminal biotin tag) pSEB-Display
pBG2Luc (GLuc-cGFP reporter) pSEB-GLuc1 (PCA assay) pSEB-GLuc2 (PCA assay) pBRS (RheoSwitch inducible)
pHRS (RheoSwitch inducible) pNRS (RheoSwitch inducible) pPRS (RheoSwitch inducible) pRV-Linker
pRV-RFP pRV-TO4 pRV-TO5 pRV-TOMT (Myc)
pRV-TOFT (Flag) pRV-TOHis (6xHis) pSCV-Flag pSCX-Flag
pSCV-His pSCX-His pSCV-Linker pSCV-BsdRFP
pSCV-TOFT (Flag) pSCV-TOHis (6xHis) pSCV-TOFc (IgG)  
CRISPR-Based HDR Vectors and Immortalization Vectors
pAAVS1-TAT2 pRosa26-TA   pMPH-TAT
pAdHD-Rosa26-TAT pAdHD-AAVS1-TAT pRosa26-TAT pMPH86 (hygro-TAg immortalizing)
pCas9gR pCas9gR pCas9gG-AAVS1  
pCas9gG pCas9gG-AAVS1-2 pCas9gG-AAVS1-3 SSR#41 (FRT)
pHDR-AAVS1 pAAVS1-TA  pAAVS1-TERT pAAVS1-TAT SSR#69 (loxP)
pHDR-Rosa26 pRosa26-TERT pCas9gG-Rosa26  
pMOK-FRTs  pHDR1  pHDR  
piggyBac Vectors
PNC2 (CMV/G418) PNC3 (CMV/G418) PNC4 (CMV/G418) PNC5 (CMV/G418)
pMPB2 (hEFH) pMPB3 (hEFH) pMPB4 (hEFH) pMPB5 (hEFH)
PBC2 (CMV/Bsd) PBC3 (CMV/Bsd) PBC4 (CMV/Bsd) PBC5 (CMV/Bsd)
pMOS4 (siRNA) pMPH (hygro) pMPN (G418) pMPZ (zeocin)
pMPH-FRT pMPH-Lox   pMPBLox (piggyBac)
pMPB (piggyBac) (siRNA) pMOS1 (siRNA) pMOS2 (siRNA) pMOS3 (siRNA)
PBC2-FLuc PBC2-cGFP PBC2Lox pBCFc (human IgG1 Fc)
       
pPBH-TREtight (Shen/Turner) pPBH-P-TREtight-eGFP-C (Shen/Turner)    
Mammalian Expression Vectors (with tags or fusion proteins)
pCMV-NK-HAC pCMV-GLuc (Gaussia Luciferase) pCMV-CHis2 (6xHis) pCMV-hFc (IgG)

pCMV-CFT (Flag)

pCMV-CRFP (red FP) pCMV-CMT2 (Myc) pCMV-CHA2 (HA)
pCMV-CHA (HA) pCMV-CFP (cyan FP) pCMV-hFc2 (IgG) pCMV-YFP (Venus)
pCMV-CHis (6xHis) pCMV-CAP (SEAP) pCMV-CePF (Cerulean) pCMV-OFP (mKO1)
pCMV-CMT (Myc)

pCMV-CFT2 (Flag)

pSOS-HUS (non-RV,Sfi I) pSOS-HUD (non-RV, Xcm I)
pHAHA pmRFP-C1    
 Inducible, Cloning, Reporter and Misc Vectors
pTOP-Luc pCAT (cloning vector) pMOLUC (cloning vector) pMOK (cloning vector)

pBV-Luc (Luc reporter)

pGST-MOLUC   pMOK1 (cloning vector)
pTHE pTRE pTLOX pMOK2 (cloning vector)

Commercial Vectors

pDisplay pEGFP-C1 pEGFP-N1 pcDNA3.1
pCMVb pGEX vectors pBlueScript II KS pUC19
pCEP4 pCMV-SPORT 6 pDNR-LIB pOTB7
pCI-neo pGL3-Control pBI-GL pBI tet vector
pCMVb pBR322 pC  
Huge Vector List      

Requested Vectors

pCS2+ pCCALL2  Seq pCCALL2-Z/EG  Seq Miami Mice      
             
             

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