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 Molecular Oncology Laboratory Protocols

     MOLab Biosafety Manual   UC Safety   E & HS  Safety Talks
     MOLab Survival Manual    Safety Online Training Modules
      MOLab "Law and Order"
     MOLab Cloning Tips     Research Tips
A.  Bacterial, DNA & Cloning Techniques
B.  PCR Techniques          
C.  RNA Techniques
D.  Protein Techniques

E.  Tissue & Cell Culture Techniques  Hood Sign-up

       Cell Culture Basics Videos  V-1  V-2  V-3  V-4  V-5 

F.  Skeletal & Stem Cell Biology & Histology Techniques 
G.  Instrument Use & Procedures 
H.  Solutions, Buffers & Miscellaneous
Animal Bookkeeping:  Tumors     Bone/Stem Cells     Rats
     Molecular Oncology Lab Graduation Checklist

 MOLab Cloning and Expression Vectors

UCMOLab Vectors     MOLab Vector Template      MOLab Construct Submission Form

Adenoviral Vectors (Classical)
pAdEasyVectorspAdTrack-TOX (eGFP)pAdTrace-TOX (mRFP)pSES1 (AdV siRNA, SfiI)
pASG (AdV shuttle w/ copGFP)pASG2 (AdV w/ 2x CMV-PA)pAdTrace-TO4  (mRFP)pSES (AdV siRNA, pSES-HUS)
pShuttle-LG (LoxP-GFP)pShuttle-RFP (mRFP)pAdTrack-TO4  (eGFP) 
pAIR (Adv RheoSwitch inducible)pAdHD-Linker(helper-dependent)pAdHD-Linker2(helper-dependent) 
pAdEasy3   

MOLab One-Step (MOS), siRNA, miRs, ncRNA reporters, and CRISPR Vectors

pAdGOS   pAdGOSdpMPB-OMIRpBOK (piggyBac)  pBOKd(GA- siRNAs)pSOK  pSOKd (GA-siRNAs)
pAdROS   pAdROSdpAdTrack-OMIR  pAdTrace-OMIRpAdTrace-OK pAdTrace-OKd(siRNAs)pSOK1 (w GFP) pSOK2 (w GFP)
pU2MIR (U6 template)pSEB-OMIRpAdTrack-OK pAdTrack-OKd(siRNAs)pB2B (template for GA-siRNAs)
pNRGLuc (ncRNA reporter)pdBiFP-Linker (ncRNA reporter)pSOHU (retroviral, HindIII/SalI) 
    
pAdTrace-miRpSEB-miRpMPB-SOS (pMPB-SOSd, H3/RI)pSEB61(EcoRI/BamHI)
pAdTrace-pmiR (MluI/BmXba...)pSEB-pmiR (MluI/BmH3...)pSEB-SOSd (HindIII/EcoRI)pSEB61D (HindIII/EcoRI)
pSEB-CamiRpSEBR-CamiRpAdTrace-SOSd (HindIII/EcoRI)pAdTrace-61(EcoRI/BamHI)
pAdTrace-CamiR  pSEB67D (HindIII/EcoRI)
 pAdTrace361pSEB361pSEH361
pSEBI   
pUCGRpU6CGRpUCC4
MOLab Modified Lentiviral Vectors (MLVs)(3rd Gen)
pMLV-LinkerpMLB-LinkerpMLB    pMLBG   pMLBRpMLB-61 (EcoRI/BamHI)
pMLH-Linker  pMLB-pmiR(Mlu/BmXba...)
pMLP-Linker pMLB-3H  pMLB-3M 
pMLN-Linker pMLB-3F  pMLB-CG 
pMLZ-Linker   
pCDF1-MCS1-EF1-copGFPpCDF1-MCS2-EF1-copGFPpCDH-CMV-MCS-EF1-Puro 
Retroviral Vectors
pSEB-C3F (C-terminal 3x Flag)pSEB-N3F (N-terminal 3x Flag)pSEB-3H (3x 6His)pSEB-3M (3x Myc)
pSEB-HUS (Sfi I) (RV siRNA = pSOS)pBGLuc (GLuc reporter)pSEB-LinkerpSEB-Flag
pSEN-FlagpSEH-FlagpSEZ-FlagpSBC-3F (CMV)
pSEH-HUS (Sfi I) (RV siRNA)pSEN-HUS (Sfi I) (RV siRNA)pSEB-HUD (Xcm I) (RV siRNA)pSEB-CG (C-terminal copGFP)
pSEB-NG (N-terminal copGFP)pSEB-CBT (C-terminal biotin tag)pSEB-NBT (N-terminal biotin tag)pSEB-Display
pBG2Luc (GLuc-cGFP reporter)pSEB-GLuc1 (PCA assay)pSEB-GLuc2 (PCA assay)pBRS (RheoSwitch inducible)
pHRS (RheoSwitch inducible)pNRS (RheoSwitch inducible)pPRS (RheoSwitch inducible)pRV-Linker
pRV-RFPpRV-TO4pRV-TO5pRV-TOMT (Myc)
pRV-TOFT (Flag)pRV-TOHis (6xHis)pSCV-FlagpSCX-Flag
pSCV-HispSCX-HispSCV-LinkerpSCV-BsdRFP
pSCV-TOFT (Flag)pSCV-TOHis (6xHis)pSCV-TOFc (IgG) 
Reversible Immortalization Vectors
pAAVS1-TAT2pRosa26-TA pMLV-TAT
pAdHD-Rosa26-TATpAdHD-AAVS1-TATpRosa26-TATpMPH-TAT
pCas9gR pCas9gRpCas9gG-AAVS1pMPH86 (hygro-TAg immortalizing)
pCas9gGpCas9gG-AAVS1-2pCas9gG-AAVS1-3 
pHDR-AAVS1pAAVS1-TA  pAAVS1-TERTpAAVS1-TATSSR#41 (FRT)
pHDR-Rosa26pRosa26-TERTpCas9gG-Rosa26SSR#69 (loxP)
pMOK-FRTs  pHDR1  pHDR 
piggyBac Vectors
PNC2 (CMV/G418)PNC3 (CMV/G418)PNC4 (CMV/G418)PNC5 (CMV/G418)
pMPB2 (hEFH)pMPB3 (hEFH)pMPB4 (hEFH)pMPB5 (hEFH)
PBC2 (CMV/Bsd)PBC3 (CMV/Bsd)PBC4 (CMV/Bsd)PBC5 (CMV/Bsd)
pMOS4 (siRNA)pMPH (hygro)pMPN (G418)pMPZ (zeocin)
pMPH-FRTpMPH-Lox pMPBLox (piggyBac)
pMPB (piggyBac) (siRNA)pMOS1 (siRNA)pMOS2 (siRNA)pMOS3 (siRNA)
PBC2-FLucPBC2-cGFPPBC2LoxpBCFc (human IgG1 Fc)
    
pPBH-TREtight (Shen/Turner)pPBH-P-TREtight-eGFP-C (Shen/Turner)  
Mammalian Expression Vectors (with tags or fusion proteins)
pCMV-NK-HACpCMV-GLuc (Gaussia Luciferase)pCMV-CHis2 (6xHis)pCMV-hFc (IgG)

pCMV-CFT (Flag)

pCMV-CRFP (red FP)pCMV-CMT2 (Myc)pCMV-CHA2 (HA)
pCMV-CHA (HA)pCMV-CFP (cyan FP)pCMV-hFc2 (IgG)pCMV-YFP (Venus)
pCMV-CHis (6xHis)pCMV-CAP (SEAP)pCMV-CePF (Cerulean)pCMV-OFP (mKO1)
pCMV-CMT (Myc)

pCMV-CFT2 (Flag)

pSOS-HUS (non-RV,Sfi I)pSOS-HUD (non-RV, Xcm I)
pHAHApmRFP-C1  
 Inducible, Cloning, Reporter and Misc Vectors
pTOP-LucpCAT (cloning vector)pMOLUC (cloning vector)pMOK (cloning vector)

pBV-Luc (Luc reporter)

pGST-MOLUCpBR322-LinkerpMOK1 (cloning vector)
pTHEpTREpTLOXpMOK2 (cloning vector)

Commercial Vectors

pDisplaypEGFP-C1pEGFP-N1pcDNA3.1
pCMVbpGEX vectorspBlueScript II KSpUC19
pCEP4pCMV-SPORT 6pDNR-LIBpOTB7
pCI-neopGL3-ControlpBI-GLpBI tet vector
pCMVbpBR322pC 
Huge Vector List   

Requested Vectors

pCS2+pCCALL2  SeqpCCALL2-Z/EG  SeqMiami Mice   
       
       

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