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 Molecular Oncology Laboratory Protocols

     MOLab Biosafety Manual   UC Safety   E & HS  Safety Talks
     MOLab Survival Manual    Safety Online Training Modules
      MOLab "Law and Order"
     MOLab Unconventional Cloning Tips
A.  Bacterial, DNA & Cloning Techniques
B.  PCR Techniques          
C.  RNA Techniques
D.  Protein Techniques

E.  Tissue & Cell Culture Techniques  Hood Sign-up

       Cell Culture Basics Videos  V-1  V-2  V-3  V-4  V-5 

F.  Skeletal & Stem Cell Biology & Histology Techniques 
G.  Instrument Use & Procedures 
H.  Solutions, Buffers & Miscellaneous
Animal Bookkeeping:  Tumors     Bone/Stem Cells     Rats
     Molecular Oncology Lab Graduation Checklist

 MOLab Cloning and Expression Vectors

UCMOLab Vectors     MOLab Vector Template      MOLab Construct Submission Form

Adenoviral Vectors (Classical)
pAdEasyVectors pAdTrack-TOX (eGFP) pAdTrace-TOX (mRFP) pSES1 (AdV siRNA, SfiI)
pASG (AdV shuttle w/ copGFP) pASG2 (AdV w/ 2x CMV-PA) pAdTrace-TO4  (mRFP) pSES (AdV siRNA, pSES-HUS)
pShuttle-LG (LoxP-GFP) pShuttle-RFP (mRFP) pAdTrack-TO4  (eGFP)  
pAIR (Adv RheoSwitch inducible) pAdHD-Linker(helper-dependent) pAdHD-Linker2(helper-dependent)  
pAdEasy3      

MOLab One-Step (MOS), miRs, ncRNA reporter, and CRISPR Vectors

pAdGOS   pAdGOSd pMPB-OMIR pBOK (piggyBac)  pBOKd(GA- siRNAs) pSOK  pSOKd (GA-siRNAs)
pAdROS   pAdROSd pAdTrack-OMIR pAdTrace-OK pAdTrace-OKd(siRNAs) pSOK1 (w GFP) pSOK2 (w GFP)
pU2MIR (U6 template) pAdTrace-OMIR pAdTrack-OK pAdTrack-OKd(siRNAs) pB2B (template for GA-siRNAs)
pNRGLuc (ncRNA reporter) pSEB-OMIR pSOHU (retroviral, HindIII/SalI) pMPB-SOS (pMPB-SOSd, H3/RI)
pAdTrace-AMO pSEB-AMO   pSEB-SOSd (HindIII/EcoRI)
pAdTrace-miR pSEB-miR   pAdTrace-SOSd  (HindIII/EcoRI)
       
pUCGR pU6CGR pUCC4  
CRISPR-Based HDR Vectors
pAdHD-Rosa26-TAT pAdHD-AAVS1-TAT    
pCas9gR pCas9gR pCas9gG pCas9gG-AAVS1   pCas9gG-Rosa26
pMOK-FRTs  pHDR1  pHDR pCas9gG-AAVS1-2 pCas9gG-AAVS1-3  
pHDR-AAVS1 pAAVS1-TA  pAAVS1-TERT pAAVS1-TAT pAAVS1-TAT2
pHDR-Rosa26 pRosa26-TA pRosa26-TERT pRosa26-TAT
Retroviral Vectors
pSEB-C3F (C-terminal 3x Flag) pSEB-N3F (N-terminal 3x Flag) pSEB-3H (3x 6His) pSEB-3M (3x Myc)
pSEB-HUS (Sfi I) (RV siRNA = pSOS) pBGLuc (GLuc reporter) pSEB-Linker pSEB-Flag
pSEN-Flag pSEH-Flag pSEZ-Flag pSBC-3F (CMV)
pSEH-HUS (Sfi I) (RV siRNA) pSEN-HUS (Sfi I) (RV siRNA) pSEB-HUD (Xcm I) (RV siRNA) pSEB-CG (C-terminal copGFP)
pSEB-NG (N-terminal copGFP) pSEB-CBT (C-terminal biotin tag) pSEB-NBT (N-terminal biotin tag) pSEB-Display
pBG2Luc (GLuc-cGFP reporter) pSEB-GLuc1 (PCA assay) pSEB-GLuc2 (PCA assay) pBRS (RheoSwitch inducible)
pHRS (RheoSwitch inducible) pNRS (RheoSwitch inducible) pPRS (RheoSwitch inducible) pRV-Linker
pRV-RFP pRV-TO4 pRV-TO5 pRV-TOMT (Myc)
pRV-TOFT (Flag) pRV-TOHis (6xHis) pSCV-Flag pSCX-Flag
pSCV-His pSCX-His pSCV-Linker pSCV-BsdRFP
pSCV-TOFT (Flag) pSCV-TOHis (6xHis) pSCV-TOFc (IgG)  
piggyBac Vectors
PNC2 (CMV/G418) PNC3 (CMV/G418) PNC4 (CMV/G418) PNC5 (CMV/G418)
PBC2-FLuc PBC2-cGFP PBC2Lox pBCFc (human IgG1 Fc)
pMPB2 (hEFH) pMPB3 (hEFH) pMPB4 (hEFH) pMPB5 (hEFH)
pMPB (piggyBac) (siRNA) pMOS1 (siRNA) pMOS2 (siRNA) pMOS3 (siRNA)
pMOS4 (siRNA) pMPH (hygro) pMPN (G418) pMPZ (zeocin)
pMPH-FRT pMPH-Lox pMPH86 (hygro-TAg immortalizing) pMPBLox (piggyBac)
PBC2 (CMV/Bsd) PBC3 (CMV/Bsd) PBC4 (CMV/Bsd) PBC5 (CMV/Bsd)
       
pPBH-TREtight (Shen/Turner) pPBH-P-TREtight-eGFP-C (Shen/Turner)    
Mammalian Expression Vectors (with tags or fusion proteins)
pCMV-NK-HAC pCMV-GLuc (Gaussia Luciferase) pCMV-CHis2 (6xHis) pCMV-hFc (IgG)

pCMV-CFT (Flag)

pCMV-CRFP (red FP) pCMV-CMT2 (Myc) pCMV-CHA2 (HA)
pCMV-CHA (HA) pCMV-CFP (cyan FP) pCMV-hFc2 (IgG) pCMV-YFP (Venus)
pCMV-CHis (6xHis) pCMV-CAP (SEAP) pCMV-CePF (Cerulean) pCMV-OFP (mKO1)
pCMV-CMT (Myc)

pCMV-CFT2 (Flag)

pSOS-HUS (non-RV,Sfi I) pSOS-HUD (non-RV, Xcm I)
pHAHA pmRFP-C1    
 Inducible, Cloning, Reporter and Misc Vectors
pTOP-Luc pCAT (cloning vector) pMOLUC (cloning vector) pMOK (cloning vector)

pBV-Luc (Luc reporter)

pGST-MOLUC   pMOK1 (cloning vector)
pTHE pTRE pTLOX pMOK2 (cloning vector)

Commercial Vectors

pDisplay pEGFP-C1 pEGFP-N1 pcDNA3.1
pCMVb pGEX vectors pBlueScript II KS pUC19
pCEP4 pCMV-SPORT 6 pDNR-LIB pOTB7
pCI-neo pGL3-Control pBI-GL pBI tet vector
pCMVb pBR322 pC  
Huge Vector List      

Requested Vectors

pCS2+ pCCALL2  Seq pCCALL2-Z/EG  Seq Miami Mice      
             
             

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